Posts Tagged: untranslated RNA

Long Noncoding RNA Discovery in Cardiovascular Disease: Decoding Form to Function

Long Noncoding RNA Discovery in Cardiovascular Disease: Decoding Form to Function

Tamer Sallam, Jaspreet Sandhu, Peter Tontonoz

Pipeline of long noncoding RNA (lncRNA) discovery and characterization. ASO indicates antisense oligonucleotide; ChiRP, chromatin isolation by RNA purification; qPCR, quantitative polymerase chain reaction; RAP, RNA antisense purification; and RIP, RNA immunoprecipitation. [Powerpoint File]

Circulating Noncoding RNAs as Biomarkers of Cardiovascular Disease and Injury

Circulating Noncoding RNAs as Biomarkers of Cardiovascular Disease and Injury

Janika Viereck, Thomas Thum

Biogenesis and function of microRNAs (miRNAs). MiRNAs are transcribed from longer precursors with protein or other noncoding gene sequences and further processed via 2 endonucleolytic processing steps. Mature miRNAs associate with Argonaute proteins (Ago2) forming the RNA-induced silencing complex (miRISC). Within this complex, miRNAs recognize their target sequence and block their expression by translational repression or degradation. MiRNAs can be released or actively secreted into the extracellular space and circulatory system stabilized in vesicles or proteinous binding partners. DGCR8, DiGeorge syndrome critical region 8; TRBP, transactivation-responsive RNA-binding protein. [Powerpoint File]

Circulating Noncoding RNAs as Biomarkers of Cardiovascular Disease and Injury

Circulating Noncoding RNAs as Biomarkers of Cardiovascular Disease and Injury

Janika Viereck, Thomas Thum

Molecular mechanism of long noncoding RNA (lncRNA) activities. LncRNAs regulate the expression of genes in the nucleus by interacting directly with DNA recruiting chromatin modifying complexes and various transcriptional regulators. Cytoplasmatic noncoding transcripts act as sponges for other transcripts like microRNAs (miRNAs) or for proteins, serve as templates for small peptide synthesis or regulate messenger RNA (mRNA) degradation and translation. These transcripts enter the bloodstream bound to proteinous carriers or incorporated into extracellular vesicles that leads to a stabilization of transcripts. Ago2, Argonaute protein 2. [Powerpoint File]